解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Pyrococcus woesei (DSM 3773) beta-galactosidase gene amplified by polymerase chain reaction was cloned into KpnI and HindIII binding sites of pET-30LIC expression plasmid. The obtained pGal2 (6785 bp) transcription vector was then transferred to Escherichia coli B121 (DE3) cells. High identity (99.9%) of DNA sequences...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02740841
更新日期:1998-12-01 00:00:00
abstract::A computer program (PCBI) was developed to quickly calculate codon bias index (CBI). PCBI can analyze a gene containing introns. The 22 preferred codons defined from Saccharomyces cerevisiae were used in PCBI as the standard to measure the CBI values. However, users can modify the preferred codons to suit each organis...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760858
更新日期:1998-10-01 00:00:00
abstract::Recombinant proteins are useful tools in biological research, drug development, and drug screening. Specially designed expression vectors have been developed to introduce cDNA for recombinant protein expression in mammalian cells. We have combined a dicistronic mRNA design for expression of the recombinant protein, us...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02745860
更新日期:1998-08-01 00:00:00
abstract::The two asparagine-linked glycosylation sites of recombinant coagulation factor VIIa have been characterized by glycosidase digestions, size-exclusion chromatography (SEC), and mass spectrometry (MS). Nine structures were characterized as core fucosylated bi-and triantennary structures with 0-3 sialic-acid residues wh...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Lactococcus lactis is industrially important microorganism used in many dairy fermentations. Numerous genes and gene expression signals from this organism have now been identified and characterized. Recently, several naturally occurring, inducible gene-expression systems have also been described in L. lactis. The main...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02760814
更新日期:1998-04-01 00:00:00
abstract::An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained b...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02752699
更新日期:1998-02-01 00:00:00
abstract::Identification of expressed sequence tags (ESTs) in large genomic segments is an important step in positional cloning and genomic mapping studies. A simple and efficient polymerase chain reaction (PCR)-based approach is described here to identify coding sequences in large genomic fragments of DNA cloned in vectors suc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02760779
更新日期:1997-12-01 00:00:00
abstract::Clinical molecular genetics has recently become recognized as a diagnostic discipline. This article covers the evolution, structure, and possible forward development of clinical molecular genetics. Topics covered include general test categories, introducing new tests, laboratory facilities, staffing and training, and ...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02752254
更新日期:1997-10-01 00:00:00
abstract::Molecular biological methods that use antibodies and nucleic acids to detect specific foodborne bacterial pathogens were scarcely known a decade and a half ago. Few scientists could have predicted that these tools of basic research would come to dominate the field of food diagnostics. Today, a large number of cleverly...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02740817
更新日期:1997-06-01 00:00:00
abstract::The monomeric bacteriophage RNA polymerases allow the synthesis of virtually any RNA molecule in unlimited quantity. In this protocol, we describe the preparation of plasmid and PCR-derived templates. A basic transcription protocol is provided with several optional modifications. The use of RNA probes in Northern blot...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02761751
更新日期:1997-04-01 00:00:00
abstract::Electroporation of cells in the presence of DNA is widely used for the introduction of transgenes either stably or transiently into bacterial, fungal, animal, and plant cells. A review of the literature shows that electroporation parameters are often reported in an incomplete or incorrect manner, forcing researchers t...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02821542
更新日期:1997-02-01 00:00:00
abstract::The sorbents with immobilized histidine as a pseudo affinity ligand with a wide specificity is described. The possibilities and relevant chemistries to use both particulate and flat or hollow fiber membranes as support matrices are discussed. The usefulness of such adsorbents for the purification of a wide variety of ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02761713
更新日期:1996-12-01 00:00:00
abstract::The simple use of nonisotopic hybridization probes to detect complementary sequences provides valuable information in a large number of research and commercial applications. In hybridization assays, the four "S's (speed, simplicity, sensitivity, and specificity) are important criteria for determining the choice of pro...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02740771
更新日期:1996-10-01 00:00:00
abstract::This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database. Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than another. Recent developments have been the World Wide Web submission tool,...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02762322
更新日期:1996-08-01 00:00:00
abstract::The term "coincidence cloning" encompasses a wide range of methodologies, the aim of which is to isolate DNA sequences which occur in both of two input DNA sources. The nature of these input DNAs may be genomic or cDNA, cloned or uncloned, and as such the far reaching applicability of the techniques can be imagined. I...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02900362
更新日期:1996-06-01 00:00:00
abstract::This article presents a simple and rapid method for removal of unincorporated label and proteins from DNA sequencing reactions by using Wizard purification resin. This method can be successfully applied for preparation of end-labeled oligonucleotides free of unincorporated label, which is important in experiments (inc...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789066
更新日期:1996-04-01 00:00:00
abstract::This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be den...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02779017
更新日期:1995-12-01 00:00:00
abstract::In this review I briefly describe the technique of protein engineering and indicate how the present state of knowledge allows proteins to be mutated to increase or decrease stability. I discuss experiments on both model proteins and those of relevance to the food industry and show how hydrophobic forces are a major dr...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02921609
更新日期:1995-10-01 00:00:00
abstract::Despite the known importance of the composition of culture media and culture conditions on Bacillus thuringiensis growth and toxicity, very few reviews are concerned with this subject. This article reviews some aspects of the microbiology of Bacillus thuringiensis, and how toxicity is affected by the composition of gr...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02907471
更新日期:1995-08-01 00:00:00
abstract::Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789331
更新日期:1995-06-01 00:00:00
abstract::Positional cloning of rare disease genes depends on the availability of highly polymorphic markers near the disease loci. The most abundant class of polymorphic markers in the human genome is CA-repeats. We have developed a strategy for the rapid isolation of highly polymorphic CA-repeats from YAC clones. Total DNA of...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789104
更新日期:1995-04-01 00:00:00
abstract::There is a growing pharmaceutical market for protein-based drugs for use in therapy and diagnosis. The rapid developments in molecular and cell biology have resulted in production of expression systems for manufacturing of recombinant proteins and monoclonal antibodies. These proteins are glycosylated when expressed i...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02745880
更新日期:1994-12-01 00:00:00
abstract::A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments. ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02789291
更新日期:1994-08-01 00:00:00
abstract::Yeast artificial chromosome (YAC) cloning systems enable the cloning of DNA stretches of 50 to well over 2000 kb. This makes it possible to study large intact regions of DNA in detail, by restriction mapping the YAC to produce a physical map and by examining the YAC for coding sequences or genes. YACs are important fo...
journal_title:Molecular biotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF02921558
更新日期:1994-04-01 00:00:00
abstract::Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral ...
journal_title:Molecular biotechnology
pub_type: 杂志文章
doi:10.1007/BF02821510
更新日期:1994-02-01 00:00:00